The HSQC methodology was optimised for use in in vivo experiments and the first reproducibility experiments were performed, with positive outcomes. Proof-of-principle experiments, using the incorporation of 13C labelled lipids into a meal, are carried out. After this validation of the methodology in vivo, and optimisation of the 13C-fatty acid dose that needs to be ingested, the dynamics of ectopic fat storage can be investigated in relation to pathophysiology and food composition.
|Scientific papers in peer-reviewed journals||2015 Effects of high-fat feeding on ectopic fat storage and postprandial lipid metabolism in mouse offspring||View summary|
|Posters||2015 Quantum coherence spectroscopy to measure 1H-[13C] -lipid signals||View summary|
|Scientific papers in peer-reviewed journals||2015 Proton magnetic resonance spectroscopy reveals increased hepatic lipid content after a single high-fat meal with no additional modulation by added protein||View summary|
|Posters||2014 Postprandial ectopic lipid storage observed after a single meal: no influence of additional protein content||View summary|