Obesity is a world-wide problem affecting the health of many people. It is not only characterised by excessive fat storage in white adipose tissue, but also as ectopic fat, present in, for example, heart, liver and muscles. Fat storage in these tissues leads to metabolic diseases such as insulin resistance, diabetes and cardiovascular disease. To date, very little is known about the causes of ectopic-fat accumulation, including the role of diet.The main goal of this project is to develop Carbon Magnetic Resonance Spectroscopy (13C-MRS) methodology to allow the tracking of 13C-labelled lipids into liver and muscle. An innovative (indirect) detection method, based on Heteronuclear Single Quantum Coherence (HSQC), has been developed and the feasibility of this method has already been demonstrated in phantom experiments.
The HSQC methodology was optimised for use in in vivo experiments and the first reproducibility experiments were performed, with positive outcomes. Proof-of-principle experiments, using the incorporation of 13C labelled lipids into a meal, are carried out. After this validation of the methodology in vivo, and optimisation of the 13C-fatty acid dose that needs to be ingested, the dynamics of ectopic fat storage can be investigated in relation to pathophysiology and food composition.
|Scientific papers in peer-reviewed journals||2015 Effects of high-fat feeding on ectopic fat storage and postprandial lipid metabolism in mouse offspring||View summary|
|Posters||2015 Quantum coherence spectroscopy to measure 1H-[13C] -lipid signals||View summary|
|Scientific papers in peer-reviewed journals||2015 Proton magnetic resonance spectroscopy reveals increased hepatic lipid content after a single high-fat meal with no additional modulation by added protein||View summary|
|Posters||2014 Postprandial ectopic lipid storage observed after a single meal: no influence of additional protein content||View summary|